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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Tissue

Detectable

Cell line

Detectable

Category

Score

Score

Score

Antibodies

Column

 
 
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APOL1
  • premium

    TISSUE

  • CELL

  • CANCER

CANCER ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

CANCER ATLASi

The cancer tissue page shows antibody staining in 20 different cancers. The assay and annotation is described here.

This page starts with information about the protein evidence and, when applicable, also protein class. Below the summary, a selection of four standard cancer tissue samples is displayed as representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional 5th image can be added as a complement.

For histological reference, visit the histological dictionary.

Gene description

Apolipoprotein L, 1

Protein class

Candidate cardiovascular disease genes, Disease related genes, Plasma proteins, Predicted membrane proteins, Predicted secreted proteins

Protein evidence

Evidence at protein level
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Pancreatic cancer

STAINING SUMMARYi

The cancer tissue summary page shows antibody staining in 20 different cancers. The assay and annotation is described here.

For each cancer, the fraction of samples with antibody staining/protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the right). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The tooltip function displays additional data for the features in the staining summary view.

Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.

At the bottom of the page, a summary of the overall protein expression pattern across the analyzed cancer tissues as well as information about literature conformity are presented.

HPA018885
CAB056156

Tissue

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Tissue

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A few cases of pancreatic and liver cancers as well as basal cells carcinomas in skin showed moderate cytoplasmic staining. The remaining malignancies were generally negative or weakly stained.

Tissue

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Tissue

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A few skin, pancreatic, ovarian and stomach cancers showed moderate to strong cytoplasmic and membranous positivity. A few cases of urothelial, renal, cervical and endometrial cancers showed moderate nuclear positivity. Remaining cancer tissues were weakly stained or negative.

GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Gene name

APOL1 (HGNC Symbol)

Synonyms

APOL

Description

Apolipoprotein L, 1 (HGNC Symbol)

Entrez gene summary

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2008]

Chromosome

22

Cytoband

q12.3

Chromosome location (bp)

36253010 - 36267530

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000100342 (version 83.38)

Entrez gene

8542

UniProt

O14791 (UniProt - Evidence at protein level)

neXtProt

NX_O14791

Antibodypedia

APOL1 antibodies

PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.

APOL1-001
APOL1-003
APOL1-009
APOL1-011
APOL1-013
APOL1-201
APOL1-202
APOL1-203

PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

APOL1-001
ENSP00000380448
ENST00000397278
O14791 [Direct mapping]
Apolipoprotein L1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005254 [chloride channel activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006869 [lipid transport]
GO:0006898 [receptor-mediated endocytosis]
GO:0008203 [cholesterol metabolic process]
GO:0008289 [lipid binding]
GO:0019835 [cytolysis]
GO:0031224 [intrinsic component of membrane]
GO:0031640 [killing of cells of other organism]
GO:0034361 [very-low-density lipoprotein particle]
GO:0034364 [high-density lipoprotein particle]
GO:0042157 [lipoprotein metabolic process]
GO:0045087 [innate immune response]
GO:0072562 [blood microparticle]
GO:1902476 [chloride transmembrane transport]
Show all
398 aa
44 kDa
Yes 1
APOL1-003
ENSP00000317674
ENST00000319136
O14791 [Direct mapping]
Apolipoprotein L1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   2TM proteins predicted by MDM
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005254 [chloride channel activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006869 [lipid transport]
GO:0006898 [receptor-mediated endocytosis]
GO:0008203 [cholesterol metabolic process]
GO:0008289 [lipid binding]
GO:0019835 [cytolysis]
GO:0031224 [intrinsic component of membrane]
GO:0031640 [killing of cells of other organism]
GO:0034361 [very-low-density lipoprotein particle]
GO:0034364 [high-density lipoprotein particle]
GO:0042157 [lipoprotein metabolic process]
GO:0045087 [innate immune response]
GO:0072562 [blood microparticle]
GO:1902476 [chloride transmembrane transport]
Show all
414 aa
45.9 kDa
No 2
APOL1-009
ENSP00000404525
ENST00000438034
B1AH94 [Direct mapping]
Apolipoprotein L1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005576 [extracellular region]
GO:0006869 [lipid transport]
GO:0008289 [lipid binding]
GO:0042157 [lipoprotein metabolic process]
Show all
118 aa
13 kDa
No 1
APOL1-011
ENSP00000391302
ENST00000427990
B1AH96 [Direct mapping]
Apolipoprotein L1
Show all
   MEMSAT3 predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005576 [extracellular region]
GO:0006869 [lipid transport]
GO:0008289 [lipid binding]
GO:0042157 [lipoprotein metabolic process]
Show all
160 aa
18.1 kDa
Yes 0
APOL1-013
ENSP00000392514
ENST00000433768
B1AH95 [Direct mapping]
Apolipoprotein L1; cDNA FLJ59906, moderately similar to Apolipoprotein-L1
Show all
   MEMSAT3 predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
141 aa
15 kDa
Yes 0
APOL1-201
ENSP00000380449
ENST00000397279
O14791 [Direct mapping]
Apolipoprotein L1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005254 [chloride channel activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006869 [lipid transport]
GO:0006898 [receptor-mediated endocytosis]
GO:0008203 [cholesterol metabolic process]
GO:0008289 [lipid binding]
GO:0019835 [cytolysis]
GO:0031224 [intrinsic component of membrane]
GO:0031640 [killing of cells of other organism]
GO:0034361 [very-low-density lipoprotein particle]
GO:0034364 [high-density lipoprotein particle]
GO:0042157 [lipoprotein metabolic process]
GO:0045087 [innate immune response]
GO:0072562 [blood microparticle]
GO:1902476 [chloride transmembrane transport]
Show all
398 aa
44 kDa
Yes 1
APOL1-202
ENSP00000411507
ENST00000422706
O14791 [Direct mapping]
Apolipoprotein L1
Show all
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   # TM segments-based
   1TM proteins predicted by MDM
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005254 [chloride channel activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006869 [lipid transport]
GO:0006898 [receptor-mediated endocytosis]
GO:0008203 [cholesterol metabolic process]
GO:0008289 [lipid binding]
GO:0019835 [cytolysis]
GO:0031224 [intrinsic component of membrane]
GO:0031640 [killing of cells of other organism]
GO:0034361 [very-low-density lipoprotein particle]
GO:0034364 [high-density lipoprotein particle]
GO:0042157 [lipoprotein metabolic process]
GO:0045087 [innate immune response]
GO:0072562 [blood microparticle]
GO:1902476 [chloride transmembrane transport]
Show all
398 aa
44 kDa
Yes 1
APOL1-203
ENSP00000388477
ENST00000426053
O14791 [Direct mapping]
Apolipoprotein L1
Show all
   MEMSAT3 predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Plasma proteins
Candidate cardiovascular disease genes
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005254 [chloride channel activity]
GO:0005515 [protein binding]
GO:0005576 [extracellular region]
GO:0005615 [extracellular space]
GO:0006869 [lipid transport]
GO:0006898 [receptor-mediated endocytosis]
GO:0008203 [cholesterol metabolic process]
GO:0008289 [lipid binding]
GO:0019835 [cytolysis]
GO:0031224 [intrinsic component of membrane]
GO:0031640 [killing of cells of other organism]
GO:0034361 [very-low-density lipoprotein particle]
GO:0034364 [high-density lipoprotein particle]
GO:0042157 [lipoprotein metabolic process]
GO:0045087 [innate immune response]
GO:0072562 [blood microparticle]
GO:1902476 [chloride transmembrane transport]
Show all
380 aa
42.2 kDa
Yes 0

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