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Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Tissue

Detectable

Cell line

Detectable

Category

Score

Score

Score

Antibodies

Column

 
 
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SMAD5
  • TISSUE

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    CELL

  • CANCER

CANCER ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

CANCER ATLASi

The cancer tissue page shows antibody staining in 20 different cancers. The assay and annotation is described here.

This page starts with information about the protein evidence and, when applicable, also protein class. Below the summary, a selection of four standard cancer tissue samples is displayed as representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional 5th image can be added as a complement.

For histological reference, visit the histological dictionary.

Gene description

SMAD family member 5

Protein class

Predicted intracellular proteins, Transcription factors

Protein evidence

Evidence at protein level
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Urothelial cancer

STAINING SUMMARYi

The cancer tissue summary page shows antibody staining in 20 different cancers. The assay and annotation is described here.

For each cancer, the fraction of samples with antibody staining/protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the right). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The tooltip function displays additional data for the features in the staining summary view.

Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.

At the bottom of the page, a summary of the overall protein expression pattern across the analyzed cancer tissues as well as information about literature conformity are presented.

HPA058931

Tissue

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Tissue

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Several cases of squamous and basal cell carcinomas, gliomas, melanomas, thyroid and urothelial cancers along with a few cases of endometrial and liver cancers showed moderate to strong cytoplasmic positivity. Remaining cancer tissues were negative.

GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Gene name

SMAD5 (HGNC Symbol)

Synonyms

Dwfc, JV5-1, MADH5

Description

SMAD family member 5 (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]

Chromosome

5

Cytoband

q31.1

Chromosome location (bp)

136132845 - 136188747

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000113658 (version 83.38)

Entrez gene

4090

UniProt

Q99717 (UniProt - Evidence at protein level)

neXtProt

NX_Q99717

Antibodypedia

SMAD5 antibodies

PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.

SMAD5-001
SMAD5-002
SMAD5-003
SMAD5-004
SMAD5-005
SMAD5-008
SMAD5-010
SMAD5-201

PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

SMAD5-001
ENSP00000441954
ENST00000545279
Q99717 [Direct mapping]
Mothers against decapentaplegic homolog 5
Q68DB7 [Target identity:100%; Query identity:100%]
Mothers against decapentaplegic homolog
Show all
Predicted intracellular proteins
Transcription factors
   beta-Hairpin exposed by an alpha/beta-scaffold
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000979 [RNA polymerase II core promoter sequence-specific DNA binding]
GO:0001503 [ossification]
GO:0001657 [ureteric bud development]
GO:0001880 [Mullerian duct regression]
GO:0002051 [osteoblast fate commitment]
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005057 [receptor signaling protein activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0006468 [protein phosphorylation]
GO:0007165 [signal transduction]
GO:0007179 [transforming growth factor beta receptor signaling pathway]
GO:0007281 [germ cell development]
GO:0009880 [embryonic pattern specification]
GO:0016021 [integral component of membrane]
GO:0030218 [erythrocyte differentiation]
GO:0030509 [BMP signaling pathway]
GO:0030618 [transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity]
GO:0031625 [ubiquitin protein ligase binding]
GO:0035556 [intracellular signal transduction]
GO:0043234 [protein complex]
GO:0045669 [positive regulation of osteoblast differentiation]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0046872 [metal ion binding]
GO:0051216 [cartilage development]
GO:0060048 [cardiac muscle contraction]
GO:0060348 [bone development]
GO:0060395 [SMAD protein signal transduction]
GO:0071141 [SMAD protein complex]
GO:0071407 [cellular response to organic cyclic compound]
GO:0071773 [cellular response to BMP stimulus]
GO:1901522 [positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus]
Show all
465 aa
52.3 kDa
No 0
SMAD5-002
ENSP00000425749
ENST00000507118
D6RIZ9 [Direct mapping]
Mothers against decapentaplegic homolog 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005667 [transcription factor complex]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0007179 [transforming growth factor beta receptor signaling pathway]
Show all
128 aa
14.7 kDa
No 0
SMAD5-003
ENSP00000424279
ENST00000511116
D6RBB4 [Direct mapping]
Mothers against decapentaplegic homolog 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005667 [transcription factor complex]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0007179 [transforming growth factor beta receptor signaling pathway]
Show all
134 aa
15.4 kDa
No 0
SMAD5-004
ENSP00000426696
ENST00000509297
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
1 aa
0.1 kDa
No 0
SMAD5-005
ENSP00000427330
ENST00000515005
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
1 aa
0.1 kDa
No 0
SMAD5-008
ENSP00000422954
ENST00000506223
D6R9D4 [Direct mapping]
Mothers against decapentaplegic homolog 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005667 [transcription factor complex]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0007179 [transforming growth factor beta receptor signaling pathway]
Show all
80 aa
8.8 kDa
No 0
SMAD5-010
ENSP00000427474
ENST00000507637
H0YAK7 [Direct mapping]
Mothers against decapentaplegic homolog 5
Show all
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0006355 [regulation of transcription, DNA-templated]
Show all
174 aa
19.1 kDa
No 0
SMAD5-201
ENSP00000446474
ENST00000545620
Q99717 [Direct mapping]
Mothers against decapentaplegic homolog 5
Q68DB7 [Target identity:100%; Query identity:100%]
Mothers against decapentaplegic homolog
Show all
Predicted intracellular proteins
Transcription factors
   beta-Hairpin exposed by an alpha/beta-scaffold
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003700 [transcription factor activity, sequence-specific DNA binding]
GO:0005057 [receptor signaling protein activity]
GO:0005515 [protein binding]
GO:0005622 [intracellular]
GO:0005634 [nucleus]
GO:0005654 [nucleoplasm]
GO:0005667 [transcription factor complex]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0006351 [transcription, DNA-templated]
GO:0006355 [regulation of transcription, DNA-templated]
GO:0007165 [signal transduction]
GO:0007179 [transforming growth factor beta receptor signaling pathway]
GO:0009880 [embryonic pattern specification]
GO:0016021 [integral component of membrane]
GO:0030509 [BMP signaling pathway]
GO:0030618 [transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity]
GO:0031625 [ubiquitin protein ligase binding]
GO:0035556 [intracellular signal transduction]
GO:0043234 [protein complex]
GO:0045893 [positive regulation of transcription, DNA-templated]
GO:0046872 [metal ion binding]
GO:0060395 [SMAD protein signal transduction]
GO:0071141 [SMAD protein complex]
GO:0071773 [cellular response to BMP stimulus]
GO:1901522 [positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus]
Show all
465 aa
52.3 kDa
No 0

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The Human Protein Atlas project is funded
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