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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:100
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019141
Antibody CAB000047
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows distinct positivity in cells of seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Sertoli cells.
Expression
RNA: detected in 14 tissues Protein: detected in 27 cell types
RNA: detected in 14 tissues Protein: detected in 8 cell types
Retrieval
HIER pH6
HIER pH9
Antibody dilution
1:500
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA019141
Antibody CAB000047
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419480)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.7
39.7
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA019141
Antibody CAB000047
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.