THE HUMAN PROTEIN ATLAS BLOG

Solid-phase cloning for high-throughput assembly

2015-12-08
DNA Expression vector High-throughput cloning

Attachment of DNA fragments onto a growing strand of DNA linked to a paramagnetic bead

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts

Simple and automated workflows for DNA assembly are necessary in large-scale projects such as the Human Protein Atlas. For this reason, we have developed two automated methods for swift and convenient high-throughput assembly of DNA into expression vector constructs.

By using paramagnetic beads as a support for the DNA, we can perform construct assembly on a liquid handler robot and furthermore eliminate the need for prior purification of PCR products by spin or gel purification...Read more


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