THE HUMAN PROTEIN ATLAS BLOG

Image of the week - Nucleoli fibrillar center

2016-07-22
Cell Atlas cells Immunofluorescence microscopy organelles

Figure 1. Staining of NOLC1 (green) with DNA (blue) and microtubules (red) in U-2 OS cells.

This week, image of the week highlights another organelle, the nucleoli fibrillar center!! This week's contribution is brought to us by Lovisa Åkesson, who works on the Subcellular protein atlas, and specializes in understanding nuclear function.

A few months ago, an excellent example of a protein located in the nucleoli rim was shown in another blog post. Today, we are diving deeper into the function of the nucleoli and specifically the fibrillar center, another nucleolar compartment.

As mentioned before, the nucleolus is a non-membrane bound structure located within the nucleus and there is usually more than one in each nucleus...Read more


Vesicles with GFP

2016-05-29
Cell Atlas cells Immunofluorescence microscopy organelles

Figure 1. Staining of RAB5C (green) with DNA (blue) in HeLa cells.

Welcome to another HPA image of the week! This week we take a look at vesicles and another type of data present in the HPA.

The protein stained in Fig 1. is an image of RAB5C. This protein is found in vesicles and specifically, in lysosomes of the cell. This sample shows HeLa human cervical adenocarcinoma cells.

These cells are actually transgenic and made to express green fluorescent protein on the RAB5C protein (RAB5Cgfp). This is a method we use to check the validity of our antibodies...Read more


Blog archive

2020
2019
2018
2017 (57)
2016 (76)
2015 (13)