We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:71
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA023527
Antibody HPA069955
Standard validation
Supported
Figure description
Immunohistochemical staining of human bronchus shows strong cytoplasmic and membranous positivity in respiratory epithelial cells.
Expression
RNA: detected in 17 tissues Protein: detected in 13 cell types
Retrieval
HIER pH6
Antibody dilution
1:100
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA023527
Antibody HPA069955
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411209)
Target mass (kDa)
24.5, 18.5, 14.8
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA023527
Antibody HPA069955
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
