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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:83
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA011868
Antibody CAB024949
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human kidney shows distinct granular positivity in tubular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 64 cell types
RNA: detected in 36 tissues Protein: detected in 62 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:700
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011868
Antibody CAB024949
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415712)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
39.9, 38.4
39.9, 38.4, 25.7, 20, 14.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA011868
Antibody CAB024949
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.