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Immunohistochemical staining of human vagina shows moderate cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human vulva/anal skin shows moderate cytoplasmic and nuclear positivity in epidermal cells.
Immunohistochemical staining of human vagina shows strong cytoplasmic and nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human esophagus shows strong cytoplasmic positivity as well as a few positive nuclei in squamous epithelial cells.
Immunohistochemical staining of human cervix, uterine shows strong nuclear and cytoplasmic positivity in squamous epithelial cells.
Expression
RNA: detected in 10 tissues Protein: detected in 14 cell types
RNA: detected in 10 tissues Protein: detected in 34 cell types
RNA: detected in 10 tissues Protein: detected in 9 cell types
RNA: detected in 10 tissues Protein: detected in 12 cell types
RNA: detected in 10 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:1800
1:250
1:175
1:50
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048341
Antibody HPA049988
Antibody HPA055992
Antibody CAB018772
Antibody CAB036006
Antibody CAB036007
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
44.9, 44.8, 44.6, 42.4, 38.5, 24.4
44.9, 44.8, 44.6, 42.4, 38.5
44.9, 44.8, 44.6, 42.4, 38.5
44.8, 44.6, 38.5
44.8, 44.6, 38.5
44.8, 44.6, 38.5
Antibody dilution
1:250
1:220
1:120
1:500
1:500
1:500
PROTEIN ARRAY
Antibody HPA048341
Antibody HPA049988
Antibody HPA055992
Antibody CAB018772
Antibody CAB036006
Antibody CAB036007
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.