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EPB41L2

ANTIBODY INFORMATION
 
 

Antibody HPA005730

  

Antibody HPA006642

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA005730   HPA006642  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 4   4  

References

 1   3  

VALIDATION SUMMARY
 
 

Antibody HPA005730

  

Antibody HPA006642

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA005730

  

Antibody HPA006642

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA006642.		  
Validated

Antibody staining overlaps with antibody HPA005730.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cell junctions.  

Antibody dilution

 1:26   1:83  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA005730

  

Antibody HPA006642

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human corpus, uterine shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human rectum shows strong membranous and cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 39 cell types		   RNA: detected in 37 tissues
Protein: detected in 62 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:1000  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA005730

  

Antibody HPA006642

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 112.6, 42.2, 30.1   112.6, 104.4, 95.5, 91, 83.8, 79.3, 76.1, 71.6, 20.2, 18.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA005730

  

Antibody HPA006642

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA005730

  

Antibody HPA006642

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 64   136  

Antigen sequence

 
ELKRNFMESTPEPRPNEWEKRRITPLSLQTQGSSHETLNIVEEKKRAEVG
KDERVITEEEVEEE
  
ESQSSLRRQKREKETSESRGISRFIPPWLKKQKSYTLVVAKDGGDKKEPT
QAVVEEQVLDKEEPLPEEQRQAKGDAEEMAQKKQEIKVEVKEEKPSVSKE
EKPSVSKVEMQPTELVSKEREEKVKETQEDKLEGGA
 

Matching transcripts

 EPB41L2-001 - ENSP00000338481 [92%]
EPB41L2-010 - ENSP00000357110 [92%]
EPB41L2-014 - ENSP00000437207 [92%]
EPB41L2-027 - ENSP00000437295 [92%]
  EPB41L2-001 - ENSP00000338481 [100%]
EPB41L2-004 - ENSP00000402041 [100%]
EPB41L2-005 - ENSP00000432803 [100%]
EPB41L2-006 - ENSP00000376222 [100%]
EPB41L2-007 - ENSP00000434576 [100%]
EPB41L2-008 - ENSP00000434308 [100%]
EPB41L2-009 - ENSP00000436641 [100%]
EPB41L2-010 - ENSP00000357110 [100%]
EPB41L2-011 - ENSP00000431988 [100%]
EPB41L2-012 - ENSP00000431647 [100%]
EPB41L2-018 - ENSP00000436348 [100%]
EPB41L2-201 - ENSP00000477544 [100%]
EPB41L2-202 - ENSP00000486691 [100%]
EPB41L2-024 - ENSP00000436871 [93%]
EPB41L2-023 - ENSP00000432013 [80%]
 

Other gene match

    
ANTIGEN VIEW
«
EPB41L2-001
EPB41L2-004
EPB41L2-005
EPB41L2-006
EPB41L2-007
EPB41L2-008
EPB41L2-009
EPB41L2-010
EPB41L2-011
EPB41L2-012
EPB41L2-014
EPB41L2-018
EPB41L2-023
EPB41L2-024
EPB41L2-027
EPB41L2-201
EPB41L2-202
»

RELEVANT PUBLICATIONS
 
 

Antibody HPA005730

  

Antibody HPA006642

  

1.

 Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51
   Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy
Stadler C et al
J Proteomics 2012;75(7):2236-51
  

2.

   A network of spectrin and plectin surrounds the actin cuffs of apical tubulobulbar complexes in the rat
Aristaeus de Asis M et al
Spermatogenesis 2013;3(3):e25733
  

3.

   Affinity proteomics reveals elevated muscle proteins in plasma of children with cerebral malaria
Bachmann J et al
PLoS Pathog 2014;10(4):e1004038
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.