EIF5 - Antibodies - The Human Protein Atlas

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EIF5

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA000867

Antibody CAB004226

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA000867

sc-28309

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA000867

Antibody CAB004226

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA000867

Antibody CAB004226

Formal validation: Genetic

Validated

Significant downregulation > 25% by both siRNA:s.

Relative fluorescence intensity (RFI)

Figure description

Whole cells of segmented cells in 10x-images

Antibody dilution

1:25

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:25

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA000867

Antibody CAB004226

Standard validation

Supported

Figure description

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 70 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA000867

Antibody CAB004226

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

49.2, 22.4, 16.5

Loading control

Total protein image

Antibody dilution

1:58

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.2, 22.4, 16.5

49.2, 22.4, 16.5, 12.4, 11.5, 7.8, 5.7

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA000867

Antibody CAB004226

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:1000

ANTIGEN INFORMATION

Antibody HPA000867

Antibody CAB004226

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

150

Antigen sequence

MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPP
TYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCP
ECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSD

Matching transcripts

EIF5-001 - ENSP00000216554 [100%]
EIF5-002 - ENSP00000376477 [100%]
EIF5-003 - ENSP00000453743 [100%]
EIF5-011 - ENSP00000453856 [100%]
EIF5-010 - ENSP00000453396 [97%]

Other gene match

ANTIGEN VIEW