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EIF5

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GENERAL INFORMATION

Gene name

 EIF5

Gene description

 Eukaryotic translation initiation factor 5

Protein class

 Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 10
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

Summary

 Mainly localized to the cytosol. In addition localized to the plasma membrane.

RNA cell category

 Expressed in all

Protein evidence

 Evidence at protein level

Main location

 Localized to the Cytosol (supported)

Additional location

 In addition localized to the Plasma membrane (supported)

siRNA

 The location(s) are Validated based on siRNA mediated gene silencing.

Mouse

 Localized to the Cytosol in mouse cells.

DATA RELIABILITY

Reliability score

 Supported based on 2 antibodies.
HPA000867 , CAB004226
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ASSAYS
RNA expression
Human cells
siRNA
Mouse cells

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all
Organ
Origin
Category
Expression
Alphabetical
Cell lines sorted after organ of phenotypic resemblance.

HUMAN CELLS

Summary

 Mainly localized to the cytosol. In addition localized to the plasma membrane.

Main location

 Cytosol (supported)

Additional location

 Plasma membrane (supported)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location

Single-cell variation

Cell cycle dependent variation
CAB004226 A-431 Cytosol
CAB004226 U-2 OS Cytosol
CAB004226 U-251 MG Cytosol
HPA000867 A-431 Cytosol
HPA000867 U-2 OS Plasma membrane
Cytosol

siRNA VALIDATION
 

Relative fluorescence intensity (RFI)

Antibody

 HPA000867

siRNA validation

 Validated: Significant downregulation > 25% by both siRNA:s.

Analysis performed in

 Whole cells of segmented cells in 10x-images

siRNA 1

siRNA 2

RFI

 77%74%

p-value

 < 0.01< 0.01
 

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail 10x

Thumbnail 40x

Antibody

Sample

Cell line
HPA000867 siRNA 1 U-2 OS
HPA000867 siRNA 2 U-2 OS
HPA000867 scrambled U-2 OS

MOUSE CELLS

Main location

 Cytosol

Mouse gene

 ENSMUSG00000021282 (version 83)

 

 

 
Toggle channels
Low
High
G1
S
G2
M
N/A

Thumbnail

Antibody

Cell line

Location
HPA000867 NIH 3T3 Cytosol

GENE INFORMATION

Gene name

 EIF5

Synonyms

Description

 Eukaryotic translation initiation factor 5 (HGNC Symbol)

Entrez gene summary

 Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al., 1996 [PubMed 8663286]).[supplied by OMIM, May 2010]

Chromosome

 14

Cytoband

 q32.32

Chromosome location (bp)

 103333544 - 103345025

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000100664 (version 83.38)

Entrez gene

 1983

UniProt

 P55010 (UniProt - Evidence at protein level)

neXtProt

 NX_P55010

Antibodypedia

 EIF5 antibodies

PROTEIN BROWSER
EIF5-001
EIF5-002
EIF5-003
EIF5-007
EIF5-008
EIF5-010
EIF5-011
EIF5-012
EIF5-013
EIF5-014

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
EIF5-001
ENSP00000216554
ENST00000216554
P55010 [Direct mapping]
Eukaryotic translation initiation factor 5
A0A024R6Q1 [Target identity:100%; Query identity:100%]
Eukaryotic translation initiation factor 5, isoform CRA_b
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0003924 [GTPase activity]
GO:0005488 [binding]
GO:0005515 [protein binding]
GO:0005525 [GTP binding]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006412 [translation]
GO:0006413 [translational initiation]
GO:0006446 [regulation of translational initiation]
GO:0008135 [translation factor activity, RNA binding]
GO:0010467 [gene expression]
GO:0044267 [cellular protein metabolic process]
GO:0044822 [poly(A) RNA binding]
Show all
 431 aa
49.2 kDa 		No 0
EIF5-002
ENSP00000376477
ENST00000392715
P55010 [Direct mapping]
Eukaryotic translation initiation factor 5
A0A024R6Q1 [Target identity:100%; Query identity:100%]
Eukaryotic translation initiation factor 5, isoform CRA_b
Show all
Predicted intracellular proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0003924 [GTPase activity]
GO:0005488 [binding]
GO:0005515 [protein binding]
GO:0005525 [GTP binding]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006412 [translation]
GO:0006413 [translational initiation]
GO:0006446 [regulation of translational initiation]
GO:0008135 [translation factor activity, RNA binding]
GO:0010467 [gene expression]
GO:0044267 [cellular protein metabolic process]
GO:0044822 [poly(A) RNA binding]
Show all
 431 aa
49.2 kDa 		No 0
EIF5-003
ENSP00000453743
ENST00000558506
P55010 [Direct mapping]
Eukaryotic translation initiation factor 5
A0A024R6Q1 [Target identity:100%; Query identity:100%]
Eukaryotic translation initiation factor 5, isoform CRA_b
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0003924 [GTPase activity]
GO:0005488 [binding]
GO:0005515 [protein binding]
GO:0005525 [GTP binding]
GO:0005737 [cytoplasm]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0006412 [translation]
GO:0006413 [translational initiation]
GO:0006446 [regulation of translational initiation]
GO:0008135 [translation factor activity, RNA binding]
GO:0010467 [gene expression]
GO:0044267 [cellular protein metabolic process]
GO:0044822 [poly(A) RNA binding]
Show all
 431 aa
49.2 kDa 		No 0
EIF5-007
ENSP00000453633
ENST00000560338
H0YMJ8 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0006413 [translational initiation]
Show all
 101 aa
11.5 kDa 		No 0
EIF5-008
ENSP00000452636
ENST00000560763
H0YK29 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0006413 [translational initiation]
Show all
 51 aa
5.7 kDa 		No 0
EIF5-010
ENSP00000453396
ENST00000558316
H0YLZ1 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0006413 [translational initiation]
Show all
 146 aa
16.5 kDa 		No 0
EIF5-011
ENSP00000453856
ENST00000558265
H0YN40 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0005737 [cytoplasm]
GO:0005886 [plasma membrane]
GO:0006413 [translational initiation]
Show all
 200 aa
22.4 kDa 		No 0
EIF5-012
ENSP00000452883
ENST00000561325
H0YM54 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0006413 [translational initiation]
Show all
 109 aa
12.4 kDa 		No 0
EIF5-013
ENSP00000452615
ENST00000559130
H0YK11 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0006413 [translational initiation]
Show all
 69 aa
7.8 kDa 		No 0
EIF5-014
ENSP00000453468
ENST00000559532
H0YM54 [Direct mapping]
Eukaryotic translation initiation factor 5
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003743 [translation initiation factor activity]
GO:0006413 [translational initiation]
Show all
 109 aa
12.4 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.