PCCB - Antibodies - The Human Protein Atlas

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PCCB

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036939

Antibody HPA036940

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA036939

HPA036940

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036939

Antibody HPA036940

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA036939

Antibody HPA036940

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036940 across 69 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036939 across 69 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 48 cell types

RNA: detected in 37 tissues
Protein: detected in 49 cell types

Retrieval

HIER pH6

Antibody dilution

1:75

1:1000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036939

Antibody HPA036940

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

61.7, 60.7, 60.5, 59.4, 58.2, 56.3, 55.7, 45.4, 32.5, 14

61.7, 60.7, 60.5, 59.4, 58.2, 56.3, 55.7, 52.4, 45.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA036939

Antibody HPA036940

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA036939

Antibody HPA036940

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

GAPVIGLNDSGGARIQEGVESLAGYADIFLRNVTASGVIPQISLIMGPCA
GGAVYSPALTDFTFMVKDTSYLFITGPDVVKSV

SSKHLCGDTNYAWPTAEIAVMGAKGAVEIIFKGHENVEAAQAEYIEKFAN
PFPAAVRGFVDDIIQPSSTRARICCDLDVL

Matching transcripts

PCCB-001 - ENSP00000251654 [100%]
PCCB-002 - ENSP00000417549 [100%]
PCCB-003 - ENSP00000419027 [100%]
PCCB-006 - ENSP00000420759 [100%]
PCCB-008 - ENSP00000420639 [100%]
PCCB-009 - ENSP00000419129 [100%]
PCCB-010 - ENSP00000420391 [100%]
PCCB-011 - ENSP00000420158 [100%]
PCCB-013 - ENSP00000417253 [100%]
PCCB-019 - ENSP00000419293 [80%]

PCCB-001 - ENSP00000251654 [100%]
PCCB-002 - ENSP00000417549 [100%]
PCCB-003 - ENSP00000419027 [100%]
PCCB-007 - ENSP00000418307 [100%]
PCCB-008 - ENSP00000420639 [100%]
PCCB-009 - ENSP00000419129 [100%]
PCCB-010 - ENSP00000420391 [100%]
PCCB-011 - ENSP00000420158 [100%]
PCCB-013 - ENSP00000417253 [100%]

Other gene match

ANTIGEN VIEW

PCCB-001	PCCB-002	PCCB-003	PCCB-006	PCCB-007	PCCB-008	PCCB-009	PCCB-010	PCCB-011	PCCB-013	PCCB-019

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.