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Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:71
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA054371
Antibody HPA057950
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows strong granular cytoplasmic positivity in cells in seminiferus ducts.
Immunohistochemical staining of human spleen shows positivity in white pulp.
Expression
RNA: detected in 4 tissues Protein: detected in 54 cell types
RNA: detected in 4 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:175
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA054371
Antibody HPA057950
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
24.1, 21.9, 21.5, 19.6, 17.5
24.1
Antibody dilution
1:100
1:240
PROTEIN ARRAY
Antibody HPA054371
Antibody HPA057950
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.