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EFR3A

ANTIBODY INFORMATION
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA022859   HPA023092   HPA023402  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 5   5   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  
Immunocytochemistry

    Supported  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Uncertain   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.  

Antibody dilution

     1:50  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA023092 across 75 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA022859 across 75 cell types.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 41 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:50   1:35  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 92.9, 88.8   92.9, 88.8   92.9, 88.8  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA022859

  

Antibody HPA023092

  

Antibody HPA023402

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 71   85   82  

Antigen sequence

 
NTSSKDNDEKIVQNAIIQTIGFFGSNLPDYQRSEIMMFIMGKVPVFGTST
HTLDISQLGDLGTRRIQIMLL
  
LLMVTSGYKAKTIVTALPGSFLDPLLSPSLMEDYELRQLVLEVMHNLMDR
HDNRAKLRGIRIIPDVADLKIKREKICRQDTSFMK
  
IAVPAFCQHVSKVIEIRTMEAPYFLPEHIFRDKCMLPKSLEKHEKDLYFL
TNKIAESLGGSGYSVERLSVPYVPQVTDEDRL
 

Matching transcripts

 EFR3A-001 - ENSP00000254624 [100%]
EFR3A-002 - ENSP00000428086 [100%]
  EFR3A-001 - ENSP00000254624 [100%]
EFR3A-002 - ENSP00000428086 [100%]
  EFR3A-001 - ENSP00000254624 [100%]
EFR3A-002 - ENSP00000428086 [100%]
 

Other gene match

      
ANTIGEN VIEW
EFR3A-001
EFR3A-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.