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Immunofluorescent staining of human cell line RT4 shows localization to vesicles.
Antibody dilution
1:6
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA004331
Standard validation
Approved
Figure description
Immunohistochemical staining of human nasopharynx shows strong membranous positivity in respiratory epithelial cells.
Expression
RNA: detected in 23 tissues Protein: detected in 26 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Not consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA004331
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY424521)
Target mass (kDa)
59.1, 59, 57.2, 34.2
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA004331
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.