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ANTIBODY INFORMATION
 
 

Antibody HPA038005

  

Antibody CAB013634

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   SDIX
  

Product name

 HPA038005   1685  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 12   4  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA038005

  

Antibody CAB013634

  
Immunocytochemistry

Uncertain   Uncertain  
Western blot

Supported   Supported  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038005

  

Antibody CAB013634

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:30   1:200  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is not consistent with literature.  

WESTERN BLOT
 
 

Antibody HPA038005

  

Antibody CAB013634

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 53.7   53.7, 50.1, 50, 34.1, 16.2, 14.5, 2.4  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA038005

  

Antibody CAB013634

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA038005

  

Antibody CAB013634

  

Antigen

 Recombinant protein fragment   Genetic immunization  

Length (aa)

 94    

Antigen sequence

 
FARHCLKFFPAQKPACVDFGASRGRAMLTFMASDSEEEVCDERTSLMSAE
SPTPRSCQEGRQGPEDGENTAQWRSQENEEDGEEDPDRYVCSGV
  

											 
																				

																		

										
											
Matching transcripts

												
																					PSEN2-002 - ENSP00000355746 [100%] 
PSEN2-201 - ENSP00000486498 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
PSEN2-002
PSEN2-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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