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Antibody staining overlaps with antibody HPA067817.
Validated
Antibody staining overlaps with antibody HPA064111.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytokinetic bridge.
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm & cytokinetic bridge.
Antibody dilution
1:86
1:200
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA064111
Antibody HPA067817
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 29 cell types
Retrieval
HIER pH6
Antibody dilution
1:450
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA064111
Antibody HPA067817
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
38.7
38.7
Antibody dilution
1:290
1:750
PROTEIN ARRAY
Antibody HPA064111
Antibody HPA067817
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
