We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & cytosol.
Antibody dilution
1:72
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039987
Antibody HPA042645
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in astrocytes.
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in a subset of cells in seminiferus ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 59 cell types
RNA: detected in 37 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:150
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039987
Antibody HPA042645
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
65.5, 23.2
65.5
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA039987
Antibody HPA042645
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
