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Immunofluorescent staining of human cell line U-251 MG shows localization to nucleus.
Immunofluorescent staining of human cell line HeLa shows localization to nucleus.
Antibody dilution
1:5
1:2000
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA018253
Antibody CAB072814
Standard validation
Approved
Figure description
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
Antibody dilution
1:25000
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018253
Antibody CAB072814
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416234)
Target mass (kDa)
74
74, 56
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA018253
Antibody CAB072814
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
