We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & actin filaments.
Antibody dilution
1:39
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA068172
Antibody HPA069843
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows strong luminal membranous positivity in renal tubules.
Immunohistochemical staining of human cerebral cortex shows positivity in neuronal processes.
Expression
RNA: detected in 37 tissues Protein: detected in 46 cell types
RNA: detected in 37 tissues Protein: detected in 47 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:600
1:600
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA068172
Antibody HPA069843
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
70.4, 67.7
70.4, 67.7
Antibody dilution
1:130
1:140
PROTEIN ARRAY
Antibody HPA068172
Antibody HPA069843
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:1550
1:1700
ANTIGEN INFORMATION
Antibody HPA068172
Antibody HPA069843
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
45
51
Antigen sequence
EPGTPFSIGRFATLTLQERRDRGAEKEHKRYHSLGNISRGGSGGS
TYLKWLPESESLFLASHASGHLYLYNVSHPCASAPPQYSLLKQGEGFSVY
A
