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CFAP45

ANTIBODY INFORMATION
 
 

Antibody HPA042204

  

Antibody HPA043618

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042204   HPA043618  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   9  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA042204

  

Antibody HPA043618

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042204

  

Antibody HPA043618

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA043618.		  
Validated

Antibody staining overlaps with antibody HPA042204.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:200   1:58  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042204

  

Antibody HPA043618

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human breast shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human nasopharynx shows strong positivity in cilia of respiratory epithelial cells.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 30 cell types		   RNA: detected in 6 tissues
Protein: detected in 11 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1700   1:500  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042204

  

Antibody HPA043618

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 65.7   65.7, 56.5, 16.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA042204

  

Antibody HPA043618

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:12000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA042204

  

Antibody HPA043618

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   83  

Antigen sequence

 
VDESLFGDIKSPAQGQSDSPIVLLRDKHTLQKTLTALGLDRKPETIQLIT
RDMVRELIVPTEDPSGESLIISPEEFERIKWAS
  
KEATMDAVMTRKKIMKQKEMVWNNNKKLSDLEEVAKERAQNLLQRANKLR
MEQEEELKDMSKIILNAKCHAIRDAQILEKQQI
 

Matching transcripts

 CFAP45-001 - ENSP00000357079 [100%]
  CFAP45-001 - ENSP00000357079 [100%]
CFAP45-003 - ENSP00000478944 [100%]
CFAP45-201 - ENSP00000403044 [100%]
 

Other gene match

    
ANTIGEN VIEW
CFAP45-001
CFAP45-003
CFAP45-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.