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Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of the Golgi apparatus in human cell line U-251 MG (HPA001677)
Scale bar represents 10µm
Golgi apparatus
The Golgi apparatus consists of several stacks of membrane cisternae with each of them containing a specific set of proteins. It is the key organelle in the secretory pathway: it receives newly synthesized proteins from the endoplasmic reticulum, modifies them and subsequently exports them to their final destination within or outside the cell.
Immunofluorescent staining
The Golgi apparatus is located next to the nucleus. Its morphology can vary between cell lines, but typically the cisternae are stacked in a ribbon-like form. Often it is spherically shaped and centered around the microtubule-organizing center, whereas in other cell lines it encircles the whole nucleus.
