Immunofluorescent images of formaldehyde-fixed cell lines are shown. Three different organelle markers are displayed as different channels in the multicolor images - nucleus stained in blue, microtubules in red and ER in yellow. The various cell structures that are demonstrated are always shown in the green channel using an antibody found in the Human Protein Atlas. The antibody id is linked to the corresponding Cell Atlas protein page. By using the "toggle channels"-buttons, the different channels can be turned on and off. Most cell structures can be highlighted in the cell illustration by hovering over them with the exception of the aggresome. Cytoplasmic bodies are highlighted as cytosol; cytokinetic bridge, midbody, midbody ring and mitotic spindle are highlighted as microtubules, cell junctions are highlighted as plasma membrane and nucleus is highlighted as nucleoplasm.
Staining of nuclear bodies in human cell line A-431 (HPA005963)
Scale bar represents 10µm
Nuclear body is a collective term for several non-membrane-bound nuclear sub-compartments, including but not limited to Cajal bodies, Gemini of Cajal bodies and promyelocytic leukemia bodies (PML). Cajal bodies are involved in RNA splicing whereas PML bodies are thought to perform a multitude of tasks including apoptosis regulation.
Nuclear bodies are visible as distinct spots in the nucleoplasm. They can vary in number and size depending on the cell line and the type of nuclear body.