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Immunofluorescent staining of human cell line PC-3 shows localization to nucleus.
Antibody dilution
1:29
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA048100
Antibody HPA050463
Standard validation
Supported
Figure description
Immunohistochemical staining of human skeletal muscle shows nuclear membranous and cytoplasmic positivity in myocytes.
Expression
RNA: detected in 35 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
Antibody dilution
1:175
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA048100
Antibody HPA050463
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY411389)
Target mass (kDa)
33.3, 13
33.3
Antibody dilution
1:150
1:125
PROTEIN ARRAY
Antibody HPA048100
Antibody HPA050463
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.