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Antibody staining overlaps with antibody HPA012571.
Validated
Antibody staining overlaps with antibody HPA010917.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:23
1:42
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA010917
Antibody HPA012571
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human gallbladder shows strong membranous positivity in glandular cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.
Expression
RNA: detected in 28 tissues Protein: detected in 39 cell types
RNA: detected in 28 tissues Protein: detected in 77 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:200
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA010917
Antibody HPA012571
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY412853)
Target mass (kDa)
25.4
25.4, 24.7, 24.3
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA010917
Antibody HPA012571
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.