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Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
Antibody dilution
1:149
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA020192
Antibody HPA024127
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 22 tissues Protein: detected in 65 cell types
RNA: detected in 22 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:90
1:500
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA020192
Antibody HPA024127
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408680)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
56.6
56.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA020192
Antibody HPA024127
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.