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Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Antibody dilution
1:45
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038721
Antibody HPA057610
Antibody HPA074097
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 36 tissues Protein: detected in 34 cell types
RNA: detected in 36 tissues Protein: detected in 66 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:75
1:10
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038721
Antibody HPA057610
Antibody HPA074097
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
53.2
53.2
53.2
Antibody dilution
1:250
1:80
1:100
PROTEIN ARRAY
Antibody HPA038721
Antibody HPA057610
Antibody HPA074097
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.