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Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Antibody dilution
1:37
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA019105
Antibody HPA019482
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA019482 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA019105 across 74 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in cartilage.
Immunohistochemical staining of human bronchus shows strong positivity in cartilage.
Expression
RNA: detected in 13 tissues Protein: detected in 5 cell types
RNA: detected in 13 tissues Protein: detected in 10 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:35
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA019105
Antibody HPA019482
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
40.2, 29.2, 22.6, 20.6, 19, 18.1, 17.2
40.2, 29.2, 22.6, 20.6, 19, 18.1, 17.2
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA019105
Antibody HPA019482
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.