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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Antibody dilution
1:137
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA011851
Antibody HPA071067
Antibody CAB011461
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 13 tissues Protein: detected in 75 cell types
RNA: detected in 13 tissues Protein: detected in 67 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:185
1:40
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA011851
Antibody HPA071067
Antibody CAB011461
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73.8
73.8, 61.4, 51, 46.6, 22.3
73.8, 61.4, 51, 46.6, 22.3
Antibody dilution
1:250
1:450
1:500
PROTEIN ARRAY
Antibody HPA011851
Antibody HPA071067
Antibody CAB011461
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.