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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Tissue

Detectable

Cell line

Detectable

Category

Score

Score

Score

Antibodies

Column

 
 
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ACAN
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    TISSUE

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CANCER ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

CANCER ATLASi

The cancer tissue page shows antibody staining in 20 different cancers. The assay and annotation is described here.

This page starts with information about the protein evidence and, when applicable, also protein class. Below the summary, a selection of four standard cancer tissue samples is displayed as representative of the overall staining pattern. From left: colorectal cancer, breast cancer, prostate cancer and lung cancer. An additional 5th image can be added as a complement.

For histological reference, visit the histological dictionary.

Gene description

Aggrecan

Protein class

Predicted intracellular proteins, Predicted secreted proteins

Protein evidence

Evidence at protein level
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARYi

The cancer tissue summary page shows antibody staining in 20 different cancers. The assay and annotation is described here.

For each cancer, the fraction of samples with antibody staining/protein expression level high, medium, low, or not detected are provided by the blue-scale color-coding (as described by the color-coding scale in the box to the right). The length of the bar represents the number of patient samples analyzed (max=12 patients). The images and annotations can be accessed by clicking on the cancer name or protein expression bar. If more than one antibody is analyzed, the tabs at the top of the staining summary section can be used to toggle between the different antibodies. The tooltip function displays additional data for the features in the staining summary view.

Next to the cancer staining data, the protein expression data of normal tissues or specific cell types corresponding to each cancer are shown and protein expression levels are indicated by the blue-scale color coding.

At the bottom of the page, a summary of the overall protein expression pattern across the analyzed cancer tissues as well as information about literature conformity are presented.

HPA038241
HPA038242
CAB016377

Tissue

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Tissue

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Renal cancers showed moderate to strong membranous and cytoplasmic immunoreactivity. Few cases of breast cancers showed moderate staining. A subset of tumor cells in few cases of cervix, endometrial, lung and skin cancers displayed moderate cytoplasmic positivity. A single case of duct carcinoma of breast exhibited strong nucleolar staining. Remaining cancer tissues were negative.

Tissue

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Tissue

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

A single case of melanoma and fraction of cells in a rare case of urothelial cancer displayed weak to moderate cytoplasmic positivity. Remaining cancer tissues were negative.

Tissue

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Tissue

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer tissues were in general negative. Cases of hepatocellular carcinomas displayed moderate positivity.

GENE INFORMATIONi

Gene information from Ensembl and Entrez, as well as links to available gene identifiers are displayed here. Information was retrieved from Ensembl if not indicated otherwise.

Gene name

ACAN (HGNC Symbol)

Synonyms

AGC1, CSPG1, CSPGCP, MSK16

Description

Aggrecan (HGNC Symbol)

Entrez gene summary

This gene is a member of the aggrecan/versican proteoglycan family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage. Mutations in this gene may be involved in skeletal dysplasia and spinal degeneration. Multiple alternatively spliced transcript variants that encode different protein isoforms have been observed in this gene. [provided by RefSeq, Jul 2008]

Chromosome

15

Cytoband

q26.1

Chromosome location (bp)

88803443 - 88875354

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000157766 (version 83.38)

Entrez gene

176

UniProt

neXtProt

Antibodypedia

ACAN antibodies

PROTEIN BROWSERi

The protein browser displays the antigen location on the target protein(s) and the features of the target protein. The tabs at the top of the protein view section can be used to switch between the different splice variants to which an antigen has been mapped.

At the top of the view, the position of the antigen (identified by the corresponding HPA identifier) is shown as a green bar. A yellow triangle on the bar indicates a <100% sequence identity to the protein target.

Under the antigens, the maximum percent sequence identity of the protein to all other proteins from other human genes is displayed, using a sliding window of 10 aa residues (HsID 10) or 50 aa residues (HsID 50) (read more).

If a signal peptide is predicted by a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius (turquoise) and/or transmembrane regions (orange) are predicted by MDM, these are displayed.

Low complexity regions are shown in yellow and InterPro regions in green. Common (purple) and unique (grey) regions between different splice variants of the gene are also displayed (read more), and at the bottom of the protein view is the protein scale.

ACAN-001
ACAN-003
ACAN-004
ACAN-005
ACAN-006
ACAN-008
ACAN-201
ACAN-202

PROTEIN INFORMATIONi

The protein information section displays alternative protein-coding transcripts (splice variants) encoded by this gene according to the Ensembl database.

The ENSP identifier links to the Ensembl website protein summary, while the ENST identifier links to the Ensembl website transcript summary for the selected splice variant. The data in the UniProt column can be expanded to show links to all matching UniProt identifiers for this protein.

The protein classes assigned to this protein are shown if expanding the data in the protein class column. Parent protein classes are in bold font and subclasses are listed under the parent class.

The Gene Ontology terms assigned to this protein are listed if expanding the Gene ontology column. The length of the protein (amino acid residues according to Ensembl), molecular mass (kDalton), predicted signal peptide (according to a majority of the signal peptide predictors SPOCTOPUS, SignalP 4.0, and Phobius) and the number of predicted transmembrane region(s) (according to MDM) are also reported.

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

ACAN-001
ENSP00000453003
ENST00000558207
Q6PID9 [Direct mapping]
Aggrecan; Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
721 aa
79.6 kDa
Yes 0
ACAN-003
ENSP00000452682
ENST00000558704
H0YK70 [Direct mapping]
Aggrecan core protein
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
156 aa
17.3 kDa
No 0
ACAN-004
ENSP00000341615
ENST00000352105
E7ENV9 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2431 aa
250.4 kDa
Yes 0
ACAN-005
ENSP00000453581
ENST00000560601
H0YMF1 [Direct mapping]
Aggrecan core protein
Show all
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005509 [calcium ion binding]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
226 aa
24.2 kDa
No 0
ACAN-006
ENSP00000453342
ENST00000561243
E7EX88 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2530 aa
261.3 kDa
Yes 0
ACAN-008
ENSP00000453499
ENST00000559004
H0YM81 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2492 aa
257.4 kDa
Yes 0
ACAN-201
ENSP00000387356
ENST00000439576
E7EX88 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0001502 [cartilage condensation]
GO:0002063 [chondrocyte development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0005604 [basement membrane]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
GO:0007507 [heart development]
GO:0030166 [proteoglycan biosynthetic process]
GO:0030199 [collagen fibril organization]
Show all
2530 aa
261.3 kDa
Yes 0
ACAN-202
ENSP00000484456
ENST00000617301
A0A087X1T7 [Direct mapping]
Aggrecan core protein
Show all
Predicted secreted proteins
   Secreted proteins predicted by MDSEC
   SignalP predicted secreted proteins
   Phobius predicted secreted proteins
   SPOCTOPUS predicted secreted proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0001501 [skeletal system development]
GO:0005515 [protein binding]
GO:0005540 [hyaluronic acid binding]
GO:0005578 [proteinaceous extracellular matrix]
GO:0007155 [cell adhesion]
GO:0007417 [central nervous system development]
Show all
2473 aa
255.6 kDa
Yes 0

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.