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Immunohistochemical staining of human gall bladder shows distinct positivity in lymphatic vessels.
Immunohistochemical staining of human breast shows distinct positivity in myoepithelial cells and lymphatic vessels.
Expression
RNA: detected in 33 tissues Protein: detected in 17 cell types
RNA: detected in 33 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:20
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA007534
Antibody HPA073453
Antibody CAB008376
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY405041)
Target mass (kDa)
24.9, 24.7
24.9, 24.7, 16.7, 16.6, 16.1, 12.4, 12.2
24.9, 24.7, 16.7, 16.6, 16.1, 12.4, 12.2
Antibody dilution
1:250
1:960
1:500
PROTEIN ARRAY
Antibody HPA007534
Antibody HPA073453
Antibody CAB008376
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.