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Immunofluorescent staining of human cell line A-431 shows localization to the Golgi apparatus & vesicles.
Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:125
1:34
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA016759
Antibody HPA018791
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human bronchus shows cytoplasmic positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows moderate cytoplasmic positivity in respiratory epithelial cells.
Expression
RNA: detected in 36 tissues Protein: detected in 22 cell types
RNA: detected in 36 tissues Protein: detected in 21 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:750
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA016759
Antibody HPA018791
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY429597)
Target mass (kDa)
70.9, 56.5
70.9, 56.5
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA016759
Antibody HPA018791
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.