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Antibody staining overlaps with antibody HPA051484. Antibody staining overlaps with antibody HPA049121.
Validated
Antibody staining overlaps with antibody HPA051484. Antibody staining overlaps with antibody HPA047369.
Validated
Antibody staining overlaps with antibody HPA049121. Antibody staining overlaps with antibody HPA047369.
Standard validation
Supported
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus & vesicles.
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus & vesicles.
Immunofluorescent staining of human cell line HEK 293 shows localization to the Golgi apparatus.
Antibody dilution
1:6
1:21
1:104
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA047369
Antibody HPA049121
Antibody HPA051484
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA051484 across 71 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA047369 across 71 cell types.
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.
Expression
RNA: detected in 37 tissues Protein: detected in 19 cell types
RNA: detected in 37 tissues Protein: detected in 38 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:1000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA047369
Antibody HPA049121
Antibody HPA051484
Standard validation
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil