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Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in cells in white pulp.
Expression
RNA: detected in 21 tissues Protein: detected in 14 cell types
Retrieval
HIER pH6
Antibody dilution
1:2000
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041566
Antibody HPA060339
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY409449)
Target mass (kDa)
46.9
46.9
Antibody dilution
1:250
1:110
PROTEIN ARRAY
Antibody HPA041566
Antibody HPA060339
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:30000
1:1200
ANTIGEN INFORMATION
Antibody HPA041566
Antibody HPA060339
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
53
33
Antigen sequence
EVTHDLLYEGYRARCLQSLARPGARDRASRSKLSRQSATEIPLPMLPLAD
TEK