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Immunohistochemical staining of human bronchus shows distinct positivity in cilia of respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong membranous positivity in respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong positivity in cilia of respiratory epithelial cells.
Expression
RNA: detected in 16 tissues Protein: detected in 21 cell types
RNA: detected in 16 tissues Protein: detected in 8 cell types
RNA: detected in 16 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:2000
1:800
1:1000
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031655
Antibody HPA031656
Antibody HPA031657
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404885)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404885)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404885)
Target mass (kDa)
30
30
30
Antibody dilution
1:250
1:250
1:250
PROTEIN ARRAY
Antibody HPA031655
Antibody HPA031656
Antibody HPA031657
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.