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Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells of Brunner's glands.
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells in Brunner's glands.
Expression
RNA: detected in 5 tissues Protein: detected in 8 cell types
RNA: detected in 5 tissues Protein: detected in 4 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:500
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036263
Antibody HPA041449
Standard validation
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY423220)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37.9
37.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA036263
Antibody HPA041449
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.