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Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Antibody dilution
1:58
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA042815
Antibody HPA077906
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of cells outside germinal center.
Expression
RNA: detected in 36 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA042815
Antibody HPA077906
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
14.3, 12.3
Antibody dilution
1:250
PROTEIN ARRAY
Antibody HPA042815
Antibody HPA077906
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.