We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA036349 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA036348 across 72 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human urinary bladder shows cytoplasmic positivity in urothelium and distinct staining of basement membrane.
Immunohistochemical staining of human urinary bladder shows moderate cytoplasmic and nuclear positivity in urothelium and distinct positivity in basement membrane.
Immunohistochemical staining of human urinary bladder shows distinct positivity in basement membrane.
Immunohistochemical staining of human urinary bladder shows distinct membranous and cytoplasmic positivity in urothelial cells.
Expression
RNA: detected in 35 tissues Protein: detected in 63 cell types
RNA: detected in 35 tissues Protein: detected in 69 cell types
RNA: detected in 35 tissues Protein: detected in 16 cell types
RNA: detected in 35 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:500
1:90
1:25
1:75
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA036348
Antibody HPA036349
Antibody CAB002422
Antibody CAB005258
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
202.2, 200.7, 195
202.2, 200.7, 195
202.2, 200.7, 195, 21.7, 12.7, 6.7
202.2, 200.7, 195, 21.7, 12.7, 6.7
Antibody dilution
1:250
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA036348
Antibody HPA036349
Antibody CAB002422
Antibody CAB005258
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.