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Gene name

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Validation

Method

Validation

Method

Validation

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Expression

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Specificity

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Specificity

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Detectable

Cell line

Detectable

Category

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Score

Score

Antibodies

Column

 
 
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CYP51A1
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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA041325

 

Antibody HPA043508

 

Provider

Atlas Antibodies
Sigma-Aldrich
  Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA041325   HPA043508  

Host species

Rabbit   Rabbit  

Clonality

pAb   pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

   

Released in version

11   11  

References

   

VALIDATION SUMMARY

 
 

Antibody HPA041325

 

Antibody HPA043508

 
Immunocytochemistry


Validated
 

Validated
 
Immunohistochemistry

Approved   Approved  
Western blot


Validated
  Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY

 
 

Antibody HPA041325

 

Antibody HPA043508

 

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA043508.
 
Validated

Antibody staining overlaps with antibody HPA041325.
 
 

Standard validation

Supported   Supported  

   

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.   Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.  

Antibody dilution

1:93   1:108  

Literature conformity

The subcellular location is supported by literature.   The subcellular location is supported by literature.  
 

Validation: Genetic

Supported

Significant downregulation 10-25% by one/two siRNA:s
   

 

Relative fluorescence intensity (RFI)

   

Figure description

Cytoplasmic region of segmented cells in 10x-images    

Antibody dilution

1:93    

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA041325

 

Antibody HPA043508

 

Standard validation

Approved   Approved  

   

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.  

Expression

RNA: detected in 37 tissues
Protein: detected in 3 cell types
  RNA: detected in 37 tissues
Protein: detected in 5 cell types
 

Retrieval

HIER pH6   HIER pH6  

Antibody dilution

1:600   1:1000  

Literature conformity

Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA041325

 

Antibody HPA043508

 

Formal validation: Genetic

Validated

Downregulation visible in one of two siRNA lanes
   

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled
   

Target mass (kDa)

57.3, 46.3    

Loading control

Total protein image    

Antibody dilution

1:205    
 

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.
  Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
 

   

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
  Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil
 

Target mass (kDa)

57.3, 46.3   57.3  

Antibody dilution

1:250   1:250  

PROTEIN ARRAY

 
 

Antibody HPA041325

 

Antibody HPA043508

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
  Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

   

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:3000   1:6000  

ANTIGEN INFORMATION

 
 

Antibody HPA041325

 

Antibody HPA043508

 

Antigen

Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

99   86  

Antigen sequence

RSQLNEKVAQLYADLDGGFSHAAWLLPGWLPLPSFRRRDRAHREIKDIFY
KAIQKRRQSQEKIDDILQTLLDATYKDGRPLTDDEVAGMLIGLLLAGQH
 
AIAFGKSPIEFLENAYEKYGPVFSFTMVGKTFTYLLGSDAAALLFNSKNE
DLNAEDVYSRLTTPVFGKGVAYDVPNPVFLEQKKML
 

Matching transcripts

CYP51A1-001 - ENSP00000003100 [100%]
CYP51A1-002 - ENSP00000406757 [100%]
  CYP51A1-001 - ENSP00000003100 [100%]
 

Other gene match

   
ANTIGEN VIEW
CYP51A1-001
CYP51A1-002
 

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The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.