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Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & intermediate filaments.
Antibody dilution
1:62
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA005575
Antibody CAB020842
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human smooth muscle shows distinct positivity in endothelial.
Immunohistochemical staining of human colon shows distinct cytoplasmic positivity in endothelium.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
RNA: detected in 37 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:250
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA005575
Antibody CAB020842
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401895)
Target mass (kDa)
35, 33, 28.7, 24.6
35, 33, 28.7, 24.6, 12.2, 12.1, 8.6, 6.8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA005575
Antibody CAB020842
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
