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CD38

ANTIBODY INFORMATION
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
   R&D Systems
  

Product name

 HPA022132   HPA052381   NCL-CD38-290   MAB24041  

Host species

 Rabbit   Rabbit   Mouse   Mouse  

Clonality

 pAb   pAb   mAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

        

Released in version

 5   12   5   5  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  
Immunocytochemistry

Approved        
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  

Validated
  

Validated
  
Western blot

Supported   Supported   Uncertain   Uncertain  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  

Standard validation

 Approved        

       

Figure description

 Immunofluorescent staining of human cell line A549 shows localization to plasma membrane.        

Antibody dilution

 1:100        

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.        

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  
 

 

    
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 41 cell lines.  
 

Formal validation: Orthogonal

    
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		    

   
 

 

    

Figure description

     Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.    
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		      

 

  
 

      

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA052381 across 44 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA022132 across 44 cell lines.		      
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		      

 

  
 

      

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA052381 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA022132 across 74 cell types.		      
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human tonsil shows strong cytoplasmic and membranous positivity in non-germinal center cells.   Immunohistochemical staining of human prostate shows strong membranous and cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in subsets of lymphoid cells.   Immunohistochemical staining of human tonsil shows strong cytoplasmic and membranous positivity in subsets of cells.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 15 cell types		   RNA: detected in 29 tissues
Protein: detected in 15 cell types		   RNA: detected in 29 tissues
Protein: detected in 8 cell types		   RNA: detected in 29 tissues
Protein: detected in 9 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:2000   1:100   1:5000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419752)		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419752)		      

Target mass (kDa)

 34.3   34.3   34.3, 17.5   34.3, 17.5  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:3000   1:8450      

ANTIGEN INFORMATION
 
 

Antibody HPA022132

  

Antibody HPA052381

  

Antibody CAB002493

  

Antibody CAB025255

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known   Recombinant protein  

Length (aa)

 102   127      

Antigen sequence

 
AACDVVHVMLNGSRSKIFDKNSTFGSVEVHNLQPEKVQTLEAWVIHGGRE
DSRDLCQDPTIKELESIISKRNIQFSCKNIYRPDKFLQCVKNPEDSSCTS
EI
  
GTTKRFPETVLARCVKYTEIHPEMRHVDCQSVWDAFKGAFISKHPCNITE
EDYQPLMKLGTQTVPCNKILLWSRIKDLAHQFTQVQRDMFTLEDTLLGYL
ADDLTWCGEFNTSKINYQSCPDWRKDC
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					CD38-001 - ENSP00000226279 [100%] 

											 
																						CD38-001 - ENSP00000226279 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CD38-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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