UPF1 - Antibodies - The Human Protein Atlas

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UPF1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA019587

Antibody HPA020857

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA019587

HPA020857

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA019587

Antibody HPA020857

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA019587

Antibody HPA020857

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Antibody dilution

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA019587

Antibody HPA020857

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA020857 across 69 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA019587 across 69 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human duodenum shows strong granular positivity in glandular cells.

Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 66 cell types

RNA: detected in 37 tissues
Protein: detected in 61 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:10

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA019587

Antibody HPA020857

Formal validation: Genetic

Validated

Downregulation visible in both siRNA lanes

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled

Target mass (kDa)

124.3, 123

Loading control

Total protein image

Antibody dilution

1:114

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

124.3, 123

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA019587

Antibody HPA020857

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA019587

Antibody HPA020857

Antigen

Recombinant protein fragment

Length (aa)

107

Antigen sequence

LEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEA
DYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTD

KVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFA
VDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKT
VLQRPLS

Matching transcripts

UPF1-001 - ENSP00000262803 [100%]
UPF1-002 - ENSP00000470142 [100%]

Other gene match

ANTIGEN VIEW

UPF1-001	UPF1-002

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.