Cell atlas - RPAP3 - The Human Protein Atlas

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RPAP3

TISSUE

CELL

CANCER

CELL ATLAS RNA EXPRESSION HUMAN CELLS siRNA

GENE/PROTEIN

Antibody validation

Dictionary
	Cytosol

Human cell
	Cytosol

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GENERAL INFORMATION
Gene name	RPAP3
Gene description	RNA polymerase II associated protein 3
Protein class	Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	3
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the cytosol.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Cytosol (approved)
siRNA	The location(s) are Validated based on siRNA mediated gene silencing.

DATA RELIABILITY
Reliability score	Approved based on 1 antibody. HPA038311
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ASSAYS

RNA expression

Human cells

siRNA

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the cytosol.

Main location

Cytosol (approved)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA038311	A-431	Cytosol
HPA038311	U-2 OS	Cytosol
HPA038311	U-251 MG	Cytosol

siRNA VALIDATION

Relative fluorescence intensity (RFI)

Antibody

HPA038311

siRNA validation

Validated: Significant downregulation > 25% by one siRNA.

Analysis performed in

Cytoplasmic region of segmented cells in 10x-images

siRNA 1

RFI

64%

p-value

< 0.01


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA038311	siRNA 1	U-2 OS
		HPA038311	scrambled	U-2 OS

GENE INFORMATION
Gene name	RPAP3 (HGNC Symbol)
Synonyms	FLJ21908, spag
Description	RNA polymerase II associated protein 3 (HGNC Symbol)
Entrez gene summary	This gene encodes an RNA polymerase II-associated protein. The encoded protein may function in transcriptional regulation and may also regulate apoptosis. Alternatively spliced transcript variants have been described. [provided by RefSeq, Mar 2009]
Chromosome	12
Cytoband	q13.11
Chromosome location (bp)	47661249 - 47706061
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000005175 (version 83.38)
Entrez gene	79657
UniProt	Q9H6T3 (UniProt - Evidence at protein level)
neXtProt	NX_Q9H6T3
Antibodypedia	RPAP3 antibodies

PROTEIN BROWSER

RPAP3-001	RPAP3-002	RPAP3-003

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
RPAP3-001 ENSP00000005386 ENST00000005386	Q9H6T3 [Direct mapping] RNA polymerase II-associated protein 3 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	665 aa 75.7 kDa	No	0
RPAP3-002 ENSP00000401823 ENST00000432584	Q9H6T3 [Direct mapping] RNA polymerase II-associated protein 3 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	506 aa 57.1 kDa	No	0
RPAP3-003 ENSP00000370024 ENST00000380650	Q9H6T3 [Direct mapping] RNA polymerase II-associated protein 3 Show all	Predicted intracellular proteins Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005515 [protein binding] Show all	631 aa 71.9 kDa	No	0

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.