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Immunofluorescent staining of human cell line A549 shows localization to vesicles.
Antibody dilution
1:78
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043257
Antibody HPA053558
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA053558 across 76 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA043257 across 76 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human fallopian tube shows strong cytopalsmic positivity in glandular cells.
Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 68 cell types
RNA: detected in 35 tissues Protein: detected in 28 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:700
1:850
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043257
Antibody HPA053558
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
35.9, 26.2
35.9
Antibody dilution
1:250
1:390
PROTEIN ARRAY
Antibody HPA043257
Antibody HPA053558
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
