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Immunohistochemical staining of human bone marrow shows strong positivity in a subset of hematopoietic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
Antibody dilution
1:325
Literature conformity
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Standard validation
Approved
Figure description
Staining observed in median eminence, circumventricular organs and interbrain.
WESTERN BLOT
Antibody HPA022812
Antibody HPA056125
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
205, 195.7
205, 195.7
Antibody dilution
1:250
1:200
PROTEIN ARRAY
Antibody HPA022812
Antibody HPA056125
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
