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ANTIBODY INFORMATION
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA001748   HPA003877   HPA071863  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ZFY - ENSG00000067646 [100%]
     ZFY - ENSG00000067646 [100%]
  

Released in version

 2   2   16  

References

   1    

VALIDATION SUMMARY
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  
Immunocytochemistry

  Supported   Supported  
Immunohistochemistry

Supported   Supported    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.  

Antibody dilution

   1:182   1:9  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

Standard validation

 Supported   Supported    

     

Figure description

 Immunohistochemical staining of human duodenum shows moderate nuclear positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 65 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:25   1:250    

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 219, 112, 85, 49, 32, 25, 17.5
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 94.4, 90.5, 85.2, 82.2, 69.9, 66.1   94.4, 90.5, 85.2   94.4, 90.5, 85.2, 82.2, 69.9, 66.1  

Antibody dilution

 1:500   1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000   1:3000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 124   76   48  

Antigen sequence

 
IGPDGHPLTVYPCMICGKKFKSRGFLKRHMKNHPEHLAKKKYHCTDCDYT
TNKKISLHNHLESHKLTSKAEKAIECDECGKHFSHAGALFTHKMVHKEKG
ANKMHKCKFCEYETAEQGLLNRHL
  
VSLAHCTVPDDVLASDITSASMSMPEHVLTGDSIHVSDVGHVGHVGHVEH
VVHDSVVEAEIVTDPLTTDVVSEEVL
  
KTHIKTKHSKEMPFKCDICLLTFSDTKEVQQHTLVHQESKTHQCLHCD
 

Matching transcripts

 ZFX-001 - ENSP00000368475 [99%]
ZFX-002 - ENSP00000304985 [99%]
ZFX-003 - ENSP00000343384 [99%]
ZFX-201 - ENSP00000368486 [99%]
ZFX-202 - ENSP00000438233 [99%]
  ZFX-001 - ENSP00000368475 [100%]
ZFX-002 - ENSP00000304985 [100%]
ZFX-003 - ENSP00000343384 [100%]
ZFX-201 - ENSP00000368486 [100%]
  ZFX-001 - ENSP00000368475 [94%]
ZFX-002 - ENSP00000304985 [94%]
ZFX-003 - ENSP00000343384 [94%]
ZFX-201 - ENSP00000368486 [94%]
ZFX-202 - ENSP00000438233 [94%]
 

Other gene match

 ZFY - ENSG00000067646 [100%]
     ZFY - ENSG00000067646 [100%]
  
ANTIGEN VIEW
ZFX-001
ZFX-002
ZFX-003
ZFX-201
ZFX-202

RELEVANT PUBLICATIONS
 
 

Antibody HPA001748

  

Antibody HPA003877

  

Antibody HPA071863

  

1.

   Knockdown of zinc finger protein X-linked inhibits prostate cancer cell proliferation and induces apoptosis by activating caspase-3 and caspase-9
Jiang H et al
Cancer Gene Ther 2012;19(10):684-9
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.