BAIAP2L1 - Antibodies - The Human Protein Atlas

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BAIAP2L1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA019484

HPA021257

HPA023874

HPA029503

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA023874.
Antibody staining overlaps with antibody HPA021257.

Validated

Antibody staining overlaps with antibody HPA023874.
Antibody staining overlaps with antibody HPA019484.

Validated

Antibody staining overlaps with antibody HPA021257.
Antibody staining overlaps with antibody HPA019484.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.

Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.

Antibody dilution

1:200

1:182

1:100

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA023874 across 74 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA019484 across 74 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human duodenum shows strong cytoplasmic, membranous and nuclear positivity in glandular cells.

Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.

Immunohistochemical staining of human stomach shows strong cytoplasmic and membranous positivity in glandular cells.

Expression

RNA: detected in 32 tissues
Protein: detected in 63 cell types

RNA: detected in 32 tissues
Protein: detected in 78 cell types

RNA: detected in 32 tissues
Protein: detected in 58 cell types

RNA: detected in 32 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:1000

1:500

1:300

1:75

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

56.9

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA019484

Antibody HPA021257

Antibody HPA023874

Antibody HPA029503

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

ERSNVVRKDYDTLSKCSPKMPPAPSGRAYTSPLIDMFNNPATAAPNSQRV
NNSTGTSEDPSLQRSVSVATGLNMMKKQKVKTIFPHTAGSN

LLSFAQGDVITLLIPEEKDGWLYGEHDVSKARGWFPSSYTKLLEENETEA
VTVPTPSPTPVRSISTVNLSEN

DYLECLSMGAAADRRADSARTTSTFKAPASKPETAAPNDANGTAKPPFLS
GENPFATVKLRPTVTNDRSAP

EEKRRFCFLVDKHCGFANHIHYYHLQSAELLNSKLPRWQETCVDAIKVPE
KIMNMIEEIKTPASTPVSGTPQASPMI

Matching transcripts

BAIAP2L1-001 - ENSP00000005260 [100%]

Other gene match

ANTIGEN VIEW

BAIAP2L1-001

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.