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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.
Immunofluorescent staining of human cell line MCF7 shows localization to the Golgi apparatus.
Antibody dilution
1:65
1:38
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA039105
Antibody HPA039828
Antibody HPA061414
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cell.
Immunohistochemical staining of human rectum shows moderate cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 64 cell types
RNA: detected in 37 tissues Protein: detected in 70 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:450
1:250
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA039105
Antibody HPA039828
Antibody HPA061414
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
84.1, 65.3, 42.9, 32.5
84.1
84.1, 65.3
Antibody dilution
1:250
1:250
1:130
PROTEIN ARRAY
Antibody HPA039105
Antibody HPA039828
Antibody HPA061414
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
