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PSMB1

ANTIBODY INFORMATION
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA029635   HPA029637   sc-67345  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 6   6   6  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  
Immunocytochemistry

Supported      
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleus.      

Antibody dilution

 1:93      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA029637 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA029635 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts and Leydig cells.   Immunohistochemical staining of human kidney shows distinct nuclear and cytoplasmic positivity in tubular cells.   Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in renal tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 75 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:500   1:100   1:180  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 26.5   26.5   26.5  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:12000   1:500    

ANTIGEN INFORMATION
 
 

Antibody HPA029635

  

Antibody HPA029637

  

Antibody CAB033911

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 84   75    

Antigen sequence

 
YQRDSFKAGGSASAMLQPLLDNQVGFKNMQNVEHVPLSLDRAMRLVKDVF
ISAAERDVYTGDALRICIVTKEGIREETVSLRKD
  
SGFHGDCLTLTKIIEARLKMYKHSNNKAMTTGAIAAMLSTILYSRRFFPY
YVYNIIGGLDEEGKGAVYSFDPVGS
  

											 
																				

																		

										
											
Matching transcripts

												
																					PSMB1-001 - ENSP00000262193 [100%] 

											 
																						PSMB1-001 - ENSP00000262193 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

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