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CDKL5

ANTIBODY INFORMATION
 
 

Antibody HPA002847

  

Antibody CAB011577

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abcam plc
  

Product name

 HPA002847   ab22453  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 2   3  

References

 2    

VALIDATION SUMMARY
 
 

Antibody HPA002847

  

Antibody CAB011577

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Supported   Supported  
Western blot

Supported   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA002847

  

Antibody CAB011577

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:182   1:200  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA002847

  

Antibody CAB011577

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.   Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.  

Expression

 RNA: detected in 33 tissues
Protein: detected in 50 cell types		   RNA: detected in 33 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:800  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA002847

  

Antibody CAB011577

  

Standard validation

 Supported

Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY421909)		    

Target mass (kDa)

 115.5, 107.5, 88.2   115.5, 107.5, 88.2, 7.8, 6.9, 2  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA002847

  

Antibody CAB011577

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA002847

  

Antibody CAB011577

  

Antigen

 Recombinant protein fragment   Synthetic peptide  

Length (aa)

 123    

Antigen sequence

 
AARANSLQLLSPQPGEQLPPEMTVARSSVKETSREGTSSFHTRQKSEGGV
YHDPHSDDGTAPKENRHLYNDPVPRRVGSFYRVPSPRPDNSFHENNVSTR
VSSLPSESSSGTNHSKRQPAFDP
  

											 
																				

																		

										
											
Matching transcripts

												
																					CDKL5-001 - ENSP00000369325 [100%] 
CDKL5-002 - ENSP00000369332 [100%] 
CDKL5-004 - ENSP00000485184 [100%] 
CDKL5-005 - ENSP00000485244 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CDKL5-001
CDKL5-002
CDKL5-004
CDKL5-005
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA002847

												
										 
																				
											
											
											
Antibody CAB011577

												
										 
																		

																	

										
											
1.

												
																						CDKL5 ensures excitatory synapse stability by reinforcing NGL-1-PSD95 interaction in the postsynaptic compartment and is impaired in patient iPSC-derived neurons

	Ricciardi S et al

	Nat Cell Biol 2012;14(9):911-23

			
											 
																						
											 
																				

																		

										
											
2.

												
																						CDKL5 and Shootin1 Interact and Concur in Regulating Neuronal Polarization

	Nawaz MS et al

	PLoS One 2016;11(2):e0148634

			Application: ICC-IF, WB				
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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